Journal of Clinical Virology
Volume 29, Issue 3 , Pages 194-202 , March 2004

Quantitative analysis of HCMV DNA load in whole blood of renal transplant patients using real-time PCR assay

  • S. Gouarin

      Affiliations

    • Laboratory of Human and Molecular Virology, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
  • ,
  • A. Vabret

      Affiliations

    • Laboratory of Human and Molecular Virology, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
  • ,
  • E. Gault

      Affiliations

    • Laboratory of Virology, Hospital Avicenne, 125 route de Stalingrad 93009 Bobigny Cedex, France
  • ,
  • J. Petitjean

      Affiliations

    • Laboratory of Human and Molecular Virology, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
  • ,
  • A. Regeasse

      Affiliations

    • Department of Medical Computer Science, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
  • ,
  • B.Hurault de Ligny

      Affiliations

    • Department of Nephrology, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
  • ,
  • F. Freymuth

      Affiliations

    • Laboratory of Human and Molecular Virology, University Hospital, Avenue G. Clemenceau, 14033 Caen Cedex, France
    • Corresponding Author InformationCorresponding author. Tel.: +33-231-272-554; fax: +33-231-272-555.

,Accepted 8 May 2003.

References 

  1. Boeckh M, Gooley TA, Myerson D, Cunningham T, Schoch G, Bowden RA. Cytomegalovirus pp65 antigenemia-guided early treatment with ganciclovir versus ganciclovir at engraftment after allogeneic marrow transplantation: a randomized double-blind study. Blood. 1996;88:4063–4071
  2. Cope AV, Sweny P, Sabin C, Rees L, Griffiths PD, Emery VC. Quantity of cytomegalovirus viruria is a major risk factor for cytomegalovirus disease after renal transplantation. J. Med. Virol. 1997;52:200–205
  3. Drouet E, Colimon R, Michelson S, Fourcade N, Niveleau A, Ducerf C, et al. Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation. J. Clin. Microbiol. 1995;33:389–394
  4. Eckart P, Brouard J, Legoff C, Freymuth F, Duhamel JF, Ryckelynck JP, et al. Virological diagnosis of cytomegalovirus in renal transplantation: comparison of three diagnostic methods: DNA in plasma by PCR, pp65 leukocytic antigenemia, and viremia. Transplant. Proc. 1996;28:2806–2807
  5. Flexman J, Kay I, Fonte R, Herrmann R, Gabbay E, Palladino S. Differences between the quantitative antigenemia assay and the cobas amplicor monitor quantitative PCR assay for detecting CMV viraemia in bone marrow and solid organ transplant patients. J. Med. Virol. 2001;64:275–282
  6. Freymuth F, Gennetay E, Petitjean J, Eugene G, Hurault de Ligny B, Ryckelynck JP, et al. Comparison of nested PCR for detection of DNA in plasma with pp65 leukocytic antigenemia procedure for diagnosis of human cytomegalovirus infection. J. Clin. Microbiol. 1994;32:1614–1618
  7. Gault E, Michel Y, Dehee A, Belabani C, Nicolas JC, Garbarg-Chenon A. Quantification of human cytomegalovirus DNA by real-time PCR. J. Clin. Microbiol. 2001;39:772–775
  8. Gleaves CA, Smith TF, Shuster EA, Pearson GR. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 1984;19:917–919
  9. Gouarin S, Gault E, Vabret A, Cointe D, Rozenberg F, Grangeot-Keros L, et al. Real-time PCR quantification of human cytomegalovirus DNA in amniotic fluid samples from mothers with primary infection. J. Clin. Microbiol. 2002;40:1767–1772
  10. Grefte JM, van der Gun BT, Schmolke S, van der Giessen M, van Son WJ, Plachter B, et al. The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection. J. Gen. Virol. 1992;73:2923–2932
  11. Griscelli F, Barrois M, Chauvin S, Lastere S, Bellet D, Bourhis JH. Quantification of human cytomegalovirus DNA in bone marrow transplant recipients by real-time PCR. J. Clin. Microbiol. 2001;39:4362–4369
  12. Guiver M, Fox AJ, Mutton K, Mogulkoc N, Egan J. Evaluation of CMV viral load using TaqMan CMV quantitative PCR and comparison with CMV antigenemia in heart and lung transplant recipients. Transplantation. 2001;71:1609–1615
  13. Kearns AM, Guiver M, James V, King J. Development and evaluation of a real-time quantitative PCR for the detection of human cytomegalovirus. J. Virol. Methods. 2001;95:121–131
  14. Laurendeau I, Bahuau M, Vodovar N, Larramendy C, Olivi M, Bieche I, et al. TaqMan PCR-based gene dosage assay for predictive testing in individuals from a cancer family with INK4 locus haploinsufficiency. Clin. Chem. 1999;45:982–986
  15. Legoff C, Hurault de Ligny B, Freymuth F, Cardineau E, Lobbedez T, Ryckelynck P. Antigenemie leucocytaire cytomegalique quantitative. Interet clinique. Presse. méd. 1995;24:534–536
  16. Machida U, Kami M, Fukui T, Kazuyama Y, Kinoshita M, Tanaka Y, et al. Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation. J. Clin. Microbiol. 2000;38:2536–2542
  17. Mazeron MC, Ferchal F, Perol Y. Rapid detection of human cytomegalovirus viraemia from small volumes of heparinized whole blood. Res. Virol. 1993;144:169–171
  18. Mengelle C et al. Quantitation of human cytomegalovirus in recipients solid organ transplants by real-time quantitative PCR and pp65 antigenemia. J Med Virol. In press.
  19. Nicholson VA, Whimbey E, Champlin R, Abi-Said D, Przepiorka D, Tarrand J, et al. Comparison of cytomegalovirus antigenemia and shell vial culture in allogeneic marrow transplantation recipients receiving ganciclovir prophylaxis. Bone Marrow Transplant. 1997;19:37–41
  20. Nitsche A, Steuer N, Schmidt CA, Landt O, Siegert W. Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA. Clin. Chem. 1999;45:1932–1937
  21. Niubo J, Perez JL, Manito N, Garcia A, Roca J, Martin R. The polymerase chain reaction as a marker of cytomegalovirus infection in heart transplant recipients. Med. Clin. (Barc). 1999;112:121–124
  22. Razonable RR, Brown RA, Espy MJ, Rivero A, Kremers W, Wilson J, et al. Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems. J. Clin. Microbiol. 2001;39:4472–4476
  23. Sanchez JL, Storch GA. Multiplex, quantitative real-time PCR assay for cytomegalovirus and human DNA. J. Clin. Microbiol. 2002;40:2381–2386
  24. Solano C, Munoz I, Gutierrez A, Farga A, Prosper F, Garcia-Conde J, et al. Qualitative plasma PCR assay (AMPLICOR CMV) test versus pp65 antigenemia assay for monitoring cytomegalovirus viremia and guiding preemptive ganciclovir therapy in allogeneic stem cell transplantation. J. Clin. Microbiol. 2001;39:3938–3941
  25. Tanabe K, Tokumoto T, Ishikawa N, Koyama I, Takahashi K, Fuchinoue S, et al. Comparative study of cytomegalovirus (CMV) antigenemia assay, polymerase chain reaction, serology, and shell vial assay in the early diagnosis and monitoring of CMV infection after renal transplantation. Transplantation. 1997;64:1721–1725
  26. Tanaka N, Kimura H, Iida K, Saito Y, Tsuge I, Yoshimi A, et al. Quantitative analysis of cytomegalovirus load using a real-time PCR assay. J. Med. Virol. 2000;60:455–462
  27. Tong CY, Cuevas LE, Williams H, Bakran A. Prediction and diagnosis of cytomegalovirus disease in renal transplant recipients using qualitative and quantitative polymerase chain reaction. Transplantation. 2000;69:985–991
  28. van der Bij W, van Son WJ, van der Berg AP, Tegzess AM, Torensma R, The TH. Cytomegalovirus (CMV) antigenemia: rapid diagnosis and relationship with CMV-associated clinical syndromes in renal allograft recipients. Transplant. Proc. 1989;21:2061–2064
  29. Weinberg A, Hodges TN, Li S, Cai G, Zamora MR. Comparison of PCR, antigenemia assay, and rapid blood culture for detection and prevention of cytomegalovirus disease after lung transplantation. J. Clin. Microbiol. 2000;38(2):768–772

PII: S1386-6532(03)00124-0

doi: 10.1016/S1386-6532(03)00124-0

Journal of Clinical Virology
Volume 29, Issue 3 , Pages 194-202 , March 2004