Journal of Clinical Virology
Volume 35, Issue 2 , Pages 135-140 , February 2006

Stability of lyophilised specimens for the molecular detection of viral DNA/RNA

  • Helen Vaughan

      Affiliations

    • UK NEQAS for Microbiology, Quality Assurance Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 208 9059890; fax: +44 208 205 1488.
  • ,
  • Victoria J Chalker

      Affiliations

    • UK NEQAS for Microbiology, Quality Assurance Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK
  • ,
  • Zoe Mee

      Affiliations

    • National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Eire, UK
  • ,
  • Anneline Rossouw

      Affiliations

    • UK NEQAS for Microbiology, Quality Assurance Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK
  • ,
  • Vivienne James

      Affiliations

    • UK NEQAS for Microbiology, Quality Assurance Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK

Received 9 September 2004 ,Revised 12 May 2005 ,Accepted 1 June 2005.

References 

  1. Biggar RJ, Janes M, Pilon R, Miotti P, Taha T, Broadhead R, et al. Virus levels in untreated African infants infected with human immunodeficiency virus type 1. J Infect Dis. 1999;180:1838–1843
  2. Brambilla D, Jennings C, Aldrovandi G, Bremer J, Comeau A, Cassol S, et al. Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision and RNA stability. J Clin Microbiol. 2003;41:1888–1893
  3. Burfoot C, Young PA, Finter NB. The thermal stability of a stabilised 17D yellow fever virus vaccine. J Biol Stand. 1977;5:173–179
  4. Cuypers HTM, Bresters D, Winkel IN, Reesink HW, Weiner AJ, Houghton M, et al. Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C Virus. J Clin Microbiol. 1992;30:3220–3224
  5. Damon, Cuypers, HTM. Detection of HCV RNA. In: Reesink, HW, editor. Hepatitis C Virus. 2nd ed., 1997; pp. 81–94.
  6. Demello JV, Snell JJS. Preparation of simulated clinical material for bacteriological examination. J Appl Bacteriol. 1985;59:421–436
  7. Devine, D. Preparation of freeze dried simulated quality assessment specimens for cytomegalovirus detection. MSc Thesis, Royal Free Hospital School of Medicine, 1993.
  8. Grieff D, Rightsel WA. An accelerated storage test for predicting the stability of a suspension of measles virus dried by sublimation in vacuo. J Immunol. 1965;94:395–400
  9. Halfon P, Khiri H, Gerolami V, Bourliere M, Feryn JM, Reynier P, et al. Impact of various handling and storage conditions on quantitative detection of hepatitis C virus RNA. J Hepatol. 1996;25:307–311
  10. Handt O, Hoss M, Krings M, Paabo S. Ancient DNA: methodological challenges. Experientia. 1994;50:524–529
  11. HPA Investigation of herpes simplex encephalitis by Idaho LightCycler™ PCR. HPA Standards Unit, Evaluations and Standards Laboratory 2003; VSOP 19i2.1.
  12. Jerome KR, Huang M-L, Wald A, Selke S, Corey L. Quantitative stability of DNA after extended storage of clinical specimens as determined by real time PCR. J Clin Microbiol. 2002;40:2609–2611
  13. Lennette EH, Schmidt NJ. Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections. 5th ed.. Washington, DC: American Public Health Association; 1979;pp. 19–21
  14. Mehaffey MA, Cook EC, Griffin CW. Preparation and stability of freeze-dried Neisseria gonorrhoeae cultures used for external quality control. J Clin Microbiol. 1984;20:1126–1129
  15. Niesters HGM. Clinical virology in real time. J Clin Virol. 2002;25:S3–S12
  16. Paabo S. Molecular cloning of ancient Egyptian Mummy DNA. Nature. 1985;314:644–645
  17. Precausta PM, Simatos D, Le Pemp M, Devaux B, Kato F. Influence of residual moisture and sealing atmosphere on viability of two freeze-dried viral vaccines. J Clin Microbiol. 1980;12:483–489
  18. Schloss L, Van Loon AM, Cinque P, Cleator G, Echevarria JM, Falk KI, et al. An international External Quality Assessment of nucleic acid amplification of herpes simplex virus 1. J Clin Virol. 2003;28:175–185
  19. Scott EM, Woodside W. Stability of pseudorabies virus during freeze drying and storage. J Clin Microbiol. 1976;4:1–5
  20. Van Vliet KE, Muir P, Echevarria JM, Klapper PE, Cleator GM, Van Loon AM. Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses. J Clin Microbiol. 2001;39:3390–3392

PII: S1386-6532(05)00151-4

doi: 10.1016/j.jcv.2005.06.001

Journal of Clinical Virology
Volume 35, Issue 2 , Pages 135-140 , February 2006