Journal of Clinical Virology
Volume 35, Issue 2 , Pages 141-146 , February 2006

QIAamp MinElute Virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs

  • Susan E. Sefers

      Affiliations

    • Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • ,
  • Jamie Rickmyre

      Affiliations

    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • ,
  • Amondrea Blackman

      Affiliations

    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • ,
  • Haijing Li

      Affiliations

    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • ,
  • Kathryn Edwards

      Affiliations

    • Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • ,
  • Yi-Wei Tang

      Affiliations

    • Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    • Corresponding Author InformationCorresponding author at: Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232-5310, USA. Tel.: +1 615 322 2035; fax: +1 615 343 8420.

Received 10 March 2005 ,Revised 23 May 2005 ,Accepted 25 May 2005.

References 

  1. Boivin G, Cote S, Dery P, De Serres G, Bergeron MG. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J Clin Microbiol. 2004;42(1):45–51
  2. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990;28(3):495–503
  3. Deng X, Li H, Tang YW. Cytokine expression in respiratory syncytial virus-infected mice as measured by quantitative reverse-transcriptase PCR. J Virol Methods. 2003;107(2):141–146
  4. Espy MJ, Rys PN, Wold AD, Uhl JR, Sloan LM, Jenkins GD, et al. Detection of herpes simplex virus DNA in genital and dermal specimens by LightCycler PCR after extraction using the IsoQuick, MagNA Pure, and BioRobot 9604 methods. J Clin Microbiol. 2001;39(6):2233–2236
  5. Hayden FG, Osterhaus AD, Treanor JJ, Fleming DM, Aoki FY, Nicholson KG, et al. Efficacy and safety of the neuraminidase inhibitor zanamivir in the treatment of influenzavirus infections. GG167 Influenza Study Group. N Engl J Med. 1997;337(13):874–880
  6. Katcher HL, Schwartz I. A distinctive property of Tth DNA polymerase: enzymatic amplification in the presence of phenol. Biotechniques. 1994;16(1):84–92
  7. Kwok S, Mack DH, Mullis KB, Poiesz B, Ehrlich G, Blair D, et al. Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J Virol. 1987;61(5):1690–1694
  8. Lai KK, Cook L, Wendt S, Corey L, Jerome KR. Evaluation of real-time PCR versus PCR with liquid-phase hybridization for detection of enterovirus RNA in cerebrospinal fluid. J Clin Microbiol. 2003;41(7):3133–3141
  9. Li H, Dummer JS, Estes WR, Meng S, Wright PF, Tang YW. Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients. J Clin Microbiol. 2003;41(1):187–191
  10. Peiris JS, Chu CM, Cheng VC, Chan KS, Hung IF, Poon LL, et al. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study. Lancet. 2003;361(9371):1767–1772
  11. Poehling KA, Griffin MR, Dittus RS, Tang YW, Holland K, Li H, et al. Bedside diagnosis of influenzavirus infections in hospitalized children. Pediatrics. 2002;110(1 Pt 1):83–88
  12. Ramers C, Billman G, Hartin M, Ho S, Sawyer MH. Impact of a diagnostic cerebrospinal fluid enterovirus polymerase chain reaction test on patient management. JAMA. 2000;283(20):2680–2685
  13. Read SJ. Recovery efficiencies on nucleic acid extraction kits as measured by quantitative LightCycler PCR. Mol Pathol. 2001;54(2):86–90
  14. Rotbart HA, Sawyer MH, Fast S, Lewinski C, Murphy N, Keyser EF, et al. Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. J Clin Microbiol. 1994;32(10):2590–2592
  15. Rovida F, Percivalle E, Zavattoni M, Torsellini M, Sarasini A, Campanini G, et al. Monoclonal antibodies versus reverse transcription-PCR for detection of respiratory viruses in a patient population with respiratory tract infections admitted to hospital. J Med Virol. 2005;75(2):336–347
  16. Skoldenberg B, Forsgren M, Alestig K, Bergstrom T, Burman L, Dahlqvist E, et al. Acyclovir versus vidarabine in herpes simplex encephalitis. Randomised multicentre study in consecutive Swedish patients. Lancet. 1984;2(8405):707–711
  17. Smalling TW, Sefers SE, Li H, Tang YW. Molecular approaches to detecting herpes simplex virus and enteroviruses in the central nervous system. J Clin Microbiol. 2002;40(7):2317–2322
  18. Tang YW, Espy MJ, Persing DH, Smith TF. Molecular evidence and clinical significance of herpesvirus coinfection in the central nervous system. J Clin Microbiol. 1997;35(11):2869–2872
  19. Tang YW, Heimgartner PJ, Tollefson SJ, Berg TJ, Rys PN, Li H, et al. A colorimetric microtiter plate PCR system detects respiratory syncytial virus in nasal aspirates and discriminates subtypes A and B. Diagn Microbiol Infect Dis. 1999;34(4):333–337
  20. Tang YW, Rys PN, Rutledge BJ, Mitchell PS, Smith TF, Persing DH. Comparative evaluation of colorimetric microtiter plate systems for detection of herpes simplex virus in cerebrospinal fluid. J Clin Microbiol. 1998;36(9):2714–2717
  21. Treanor JJ, Hayden FG, Vrooman PS, Barbarash R, Bettis R, Riff D, et al. Efficacy and safety of the oral neuraminidase inhibitor oseltamivir in treating acute influenza: a randomized controlled trial. US Oral Neuraminidase Study Group. JAMA. 2000;283(8):1016–1024
  22. Whitley RJ, Alford CA, Hirsch MS, Schooley RT, Luby JP, Aoki FY, et al. Vidarabine versus acyclovir therapy in herpes simplex encephalitis. N Engl J Med. 1986;314(3):144–149

☆ This study was presented in part at the 20th Annual Meeting of the Pan American Society for Clinical Virology, Clearwater Beach, FL, USA, 25–28 April 2004.

PII: S1386-6532(05)00152-6

doi: 10.1016/j.jcv.2005.05.011

Journal of Clinical Virology
Volume 35, Issue 2 , Pages 141-146 , February 2006

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