Journal of Clinical Virology
Volume 35, Issue 2 , Pages 193-196 , February 2006

PCR detection of viral DNA in serum as an ancillary analysis for the diagnosis of acute mononucleosis-like syndrome due to human cytomegalovirus (HCMV) in immunocompetent patients

Received 23 February 2005 ,Revised 16 May 2005 ,Accepted 11 July 2005.

References 

  1. Alberola J, Tamarit A, Igual R, Navarro D. Early neutralizing and glycoprotein B (gB)-specific antibody responses to human cytomegalovirus (HCMV) in immunocompetent individuals with distinct clinical presentations of primary HCMV infection. J Clin Virol. 2000;16:113–122
  2. Bodeus M, Feyder S, Goubau P. Avidity of IgG antibodies distinguishes primary from non-primary cytomegalovirus infection in pregnant women. Clin Diagn Virol. 1998;9:9–16
  3. Boeckh M, Boivin G. Quantitaton of cytomegalovirus: methodologic aspects and clinical applications. Clin Microbiol Rev. 1998;11:533–554
  4. Daiminger A, Bader U, Eggers M, Lazzarotto T, Enders G. Evaluation of two novel enzyme immunoassays using recombinant antigens to detect cytomegalovirus-specific immunoglobulin M in sera from pregnant women. J Clin Virol. 1999;13:161–171
  5. Deyi YM, Goubau P, Bodeus M. False-positive IgM antibody tests for cytomegalovirus in patients with acute Epstein-Barr virus infection. Eur J Clin Microbiol Infect Dis. 2000;19:557–560
  6. Grangeot-Keros L, Mayaux MP, Lebon P, Freymuth F, Eugene G, Stricker R, et al. Value of cytomegalovirus (CMV) IgG avidity index for the diagnosis of primary CMV infection in pregnant women. J Infect Dis. 1997;175:944–946
  7. Ho M. Cytomegalovirus. Biology and infection. 2nd ed.. New York: Plenum Medical Book; 1991;
  8. Kangro HO, Griffiths PD, Huber TJ, Heath RB. Specific IgM class antibody production following infection with cytomegalovirus. J Med Virol. 1982;10:203–212
  9. Lang D, Vornhagen R, Rothe M, Hinderer W, Sonnebron HH, Platcher B. Cross-reactivity of Epstein-Barr virus specific immunoglobulin M antibodies with cytomegalovirus antigens containing glycine monopolymers. Clin Diagn Lab Immunol. 2001;8:747–756
  10. Nelson CT, Istas AS, Wilkerson MK, Demmler GJ. PCR detection of cytomegalovirus DNA in serum as a diagnostic test for congenital cytomegalovirus infection. J Clin Microbiol. 1995;33:3317–3318
  11. Revello MG, Lilleri D, Zavattoni M, Stronati M, Bollani L, Middeldorp JM, et al. Human cytomegalovirus immediate-early messenger RNA in blood of pregnant women with primary infection and of congenitally infected newborns. J Infect Dis. 2001;184:1078–1081
  12. Revello MG, Zavattoni M, Sarasini A, Percivalle E, Simoncini L, Gerna G. Human cytomegalovirus in blood of immunocompetent persons during primary infection: implications for pregnancy. J Infect Dis. 1998;177:1170–1175
  13. Rothe M, Pepperl-Klindworth S, Lang D, Vornhagen R, Hinderer W, Weise K, et al. An antigen fragment encompassing the AD2 domains of glycoprotein B from two different strains is sufficient for differentiation of primary vs. recurrent human cytomegalovirus infection by ELISA. J Med Virol. 2001;65:719–729
  14. Sipewa MJ, Goubau P, Bodéus M. Evaluation of a cytomegalovirus glycoprotein B recombinant enzyme immunoassay to discriminate between a recent and a past infection. J Clin Microbiol. 2002;40:3689–3693
  15. Solano C, Munoz I, Gutierrez A, Farga A, Prosper F, Garcia-Conde J, et al. Qualitative plasma PCR assay (AMPLICOR CMV test) versus pp65 antigenemia assay for monitoring cytomegalovirus viremia and guiding preemptive ganciclovir therapy in allogeneic stem cell transplantation. J Clin Microbiol. 2001;39:3938–3941
  16. Steininger C, Kundi M, Kletzmayr J, Aberle SW, Popow-Kraupp T. Antibody maturation and viremia after primary cytomegalovirus infection, in immunocompetent patients and kidney-transplant patients. J Infect Dis. 2004;190:1908–1912
  17. Zanghellini F, Bopana SB, Emery VC, Griffiths PD, Pass RF. Asympthomatic primary cytomegalovirus infection: virologic and immunologic features. J Infect Dis. 1999;180:702–707

PII: S1386-6532(05)00209-X

doi: 10.1016/j.jcv.2005.07.008

Journal of Clinical Virology
Volume 35, Issue 2 , Pages 193-196 , February 2006