Journal of Clinical Virology
Volume 35, Issue 2 , Pages 197-200 , February 2006

Increased levels of HIV RNA detected in samples with viral loads close to the detection limit collected in Plasma Preparation Tubes™ (PPT)

  • Brigitte P. Griffith

      Affiliations

    • VA CT Virology Reference Laboratory, VA Connecticut Healthcare System, West Haven, CT 06516, USA
    • Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA
    • Corresponding Author InformationCorresponding author at: VA CT Virology Reference Laboratory, VA Connecticut Healthcare System, 950 Campbell Avenue, West Haven, CT 06516, USA. Tel.: +1 203 932 5711x3504; fax: +1 203 937 3893.
    • ,
  • Donald R. Mayo

      Affiliations

    • VA CT Virology Reference Laboratory, VA Connecticut Healthcare System, West Haven, CT 06516, USA
    • Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA

Received 16 May 2005 ,Revised 1 August 2005 ,Accepted 15 August 2005.

References 

  1. Brambilla D, Reichelderfer PS, Bremer JW, et al. The contribution of assay variation and biological variation to the total variability of plasma HIV-1 RNA measurements. AIDS. 1999;13:2269–2279
  2. Brambilla D, Granger S, Bremer J. Variation in RNA assays at low RNA concentration. In: Proceedings of the Seventh Conference on Retrovirus and Opportunistic Infections. 2000;(Abstract 774)
  3. Griffith BP, Rigsby MO, Garner RB, Gordon MM, Chacko TM. Comparison of the Amplicor HIV-1 monitor test and the nucleic acid sequence-based amplification assay for quantitation of HIV RNA in plasma, serum and plasma subjected to freeze–thaw cycles. J Clin Microbiol. 1997;35:3288–3291
  4. Holodniy M, Mole L, Yen-Lieberman B, et al. Comparative stabilities of quantitative human immunodeficiency virus RNA in plasma samples collected in VACUTAINER CPT, VACUTAINER PPT, and standard VACUTAINER tubes. J Clin Microbiol. 1995;33:1562–1566
  5. Holodniy M, Rainen L, Herman S, Yen-Lieberman B. Stability of plasma human immunodeficiency virus load in Vacutainer PPT plasma preparation tubes during overnight shipment. J Clin Microbiol. 2000;38:323–326
  6. Lew J, Reichelderfer P, Fowler M, et al. Determinations of levels of human immunodeficiency virus type 1 RNA in plasma: reassessment of parameters affecting outcome. J Clin Microbiol. 1998;36:1471–1479
  7. Mallen MM, Veselenak II, Oates CW, et al. Comparison of VACUTAINER Brand PPT tubes with standard EDTA tubes using the Roche Amplicor HIV-1 Monitor standard and ultrasensitive procedures. In: Proceedings of the 39th ICAAC. 1999;(Abstract 1578)
  8. Murphy R, Berzins B, Leake A, et al. Effect of plasma preparation tubes (PPT) vs standard EDTA tubes on viral “blips” in patients with optimally suppressed plasma HIV-1 RNA. In: Proceedings of the 12th Conference on Retroviruses and Opportunistic Infections. 2005;(Abstract U-130)
  9. Rainen L, Salimnia H, Fairfax M, Fernandez H, Brown M. Sample handling parameters affecting performance of BD Vacutainer® PPT™ and Plus K2 EDTA tubes with the Roche Amplicor™ and Cobas Amplicor™ HIV-1 Monitor® test, v1.5 ultrasensitive and standard specimen processing procedures. BD Diagnostics recommendations, March 3, 2005.
  10. Sabino EC, Gaburo N, Leite JP, Viecili M, Rosenfeld LG. Platelets may affect detection and quantitation of HIV RNA in plasma samples with low viral loads. JAIDS. 2004;37:1432–1433
  11. Squires K, Lazzarin A, Gatell JM, et al. Comparison of once-daily atazanavir with efivarenz, each in combination with fixed-dose zidovudine and lamivudine, as initial therapy for patients infected with HIV. JAIDS. 2004;36:1011–1019

PII: S1386-6532(05)00244-1

doi: 10.1016/j.jcv.2005.08.008

Journal of Clinical Virology
Volume 35, Issue 2 , Pages 197-200 , February 2006

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