Journal of Clinical Virology
Volume 40, Issue 2 , Pages 129-134, October 2007

Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus® method☆

  • Yi-Wei Tang

      Affiliations

    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
    • Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
    • Corresponding Author InformationCorresponding author at: Molecular Infectious Diseases Laboratory, 4605 TVC, Vanderbilt University Hospital, Tennessee 37232-5310, United States. Tel.: +1 615 322 2035; fax: +1 615 343 8420.
    • ,
  • Hatim T. Allawi

      Affiliations

    • Third Wave Technologies, Inc., Madison, WI 53719, United States
    • ,
  • Marlene DeLeon-Carnes

      Affiliations

    • National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States
    • ,
  • Haijing Li

      Affiliations

    • Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
    • ,
  • Stephen P. Day

      Affiliations

    • Third Wave Technologies, Inc., Madison, WI 53719, United States
    • ,
  • D. Scott Schmid

      Affiliations

    • National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States

Received 16 March 2007; received in revised form 13 July 2007; accepted 18 July 2007. published online 29 August 2007.

Abstract 

We report the use of a prototype Invader Plus® method (Third Wave Technologies, Inc., Madison, WI) for the qualitative detection of varicella-zoster virus (VZV) and differentiation of wild-type and Oka vaccine VZV. The analytical sensitivity of the VZV Invader Plus reagents is at 10 copies per reaction. A total of 174 skin and mucous swab specimens were used to validate the assay's performance. The sensitivity and specificity were 98.3% and 98.1%, respectively, in comparison to a PCR-EIA assay. A perfect 100% agreement was obtained when VZV wild-type and vaccine differentiation was performed on 54 VZV-positive swab specimens against an allele-specific FRET real-time assay. The Invader Plus method provides another reliable tool for qualitative detection of VZV and differentiation of wild-type and vaccine virus.

Keywords: Varicella-zoster virus, Invader Plus® method, Oka varicella vaccine

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☆ The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the funding agencies.

PII: S1386-6532(07)00256-9

doi:10.1016/j.jcv.2007.07.007

Journal of Clinical Virology
Volume 40, Issue 2 , Pages 129-134, October 2007

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