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Volume 45, Issue 3 , Pages 203-204 , July 2009

Detection of novel influenza A(H1N1) virus by real-time RT-PCR

David M. Whiley
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
- Corresponding author at: Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Brisbane, Queensland, Australia. Tel.: +61 7 3636 1623; fax: +61 7 3636 1401.
Seweryn Bialasiewicz
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
Cheryl Bletchly
- Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia
Cassandra E. Faux
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
Bruce Harrower
- Public Health Virology, Forensic and Scientific Services, Coopers Plains, Brisbane, Queensland, Australia
Allan R. Gould
- Therapeutics and Molecular Mapping Unit, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
Stephen B. Lambert
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
Graeme R. Nimmo
- Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia
Michael D. Nissen
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
- Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia
Theo P. Sloots
- Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia
- Clinical Medical Virology Centre, University of Queensland, Queensland, Australia
- Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia

Received 20 May 2009 ,Accepted 22 May 2009.

References

Whiley DM, Lambert SB, Bialasiewicz S, Goire N, Nissen MD, Sloots TP. False-negative results in nucleic acid amplification tests-do we need to routinely use two genetic targets in all assays to overcome problems caused by sequence variation?. Crit Rev Microbiol. 2008;34:71–76
- CrossRef
Whiley DM, Sloots TP. A 5′-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1. Diagn Microbiol Infect Dis. 2005;53:335–337
World Health Organisation, Influenza A(H1N1) Update 23. Accessed 9 May, 2009, http://www.who.int/csr/don/2009_05_09/en/index.htm.