Journal of Clinical Virology
Volume 45, Issue 3 , Pages 205-207, July 2009

Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)

  • K.H. Chan

      Affiliations

    • Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    • ,
  • S.T. Lai

      Affiliations

    • Department of Medicine, Princess Margaret Hospital, Hong Kong Special Administrative Region, China
    • ,
  • L.L.M. Poon

      Affiliations

    • Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    • ,
  • Y. Guan

      Affiliations

    • Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    • ,
  • K.Y. Yuen

      Affiliations

    • Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    • ,
  • J.S.M. Peiris

      Affiliations

    • Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China
    • HKU-Pasteur Research Centre, Hong Kong Special Administrative Region, China
    • Corresponding Author InformationCorresponding author at: Department of Microbiology, University Pathology Building, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China. Tel.: +852 2855 4888; fax: +852 2855 1241.

Received 22 May 2009; accepted 22 May 2009. published online 19 June 2009.

Abstract 

Background

A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic.

Objectives

Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1.

Study design

Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods.

Results

The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID50) log103.3–4.7 was comparable with that of seasonal influenza (TCID50 log104.0–4.5).

Conclusion

The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.

Keywords: Diagnostics, Antigen detection, Point-of care, Pandemic, Influenza, H1N1

 

PII: S1386-6532(09)00243-1

doi:10.1016/j.jcv.2009.05.034

Journal of Clinical Virology
Volume 45, Issue 3 , Pages 205-207, July 2009

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