Journal of Clinical Virology
Volume 46, Issue 4 , Pages 314-317 , December 2009

Microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus

  • Ann R. Falsey

      Affiliations

    • Department of Medicine, Rochester General Hospital, Rochester, NY, United States
    • Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, United States
    • Corresponding Author InformationCorresponding author at: Rochester General Hospital, 1425 Portland Avenue, #246, Rochester, NY 14621-3001, United States. Tel.: +1 585 922 4339; fax: +1 585 922 5168.
    • ,
  • Maria A. Formica

      Affiliations

    • Department of Medicine, Rochester General Hospital, Rochester, NY, United States
    • ,
  • Edward E. Walsh

      Affiliations

    • Department of Medicine, Rochester General Hospital, Rochester, NY, United States
    • Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, United States

Received 7 July 2009 ,Revised 10 September 2009 ,Accepted 11 September 2009.

References 

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  2. Kahn JS. Epidemiology of human metapneumovirus. Clin Microbiol Rev. 2006;19(3):546–557
  3. Walsh EE, Peterson DR, Falsey AR. Human metapneumovirus infections in adults: another piece of the puzzle. Arch Intern Med. 2008;168:2489–2496
  4. Herfst S, Fouchier RA. Vaccination approaches to combat human metapneumovirus lower respiratory tract infections. J Clin Virol. 2008;41(1):49–52
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  6. de Graaf M, Herfst S, Schrauwen EJ, van den Hoogen BG, Osterhaus AD, Fouchier RA. An improved plaque reduction virus neutralization assay for human metapneumovirus. J Virol Methods. 2007;143(2):169–174
  7. Biacchesi S, Skiadopoulos MH, Yang L, Murphy BR, Collins PL, Buchholz UJ. Rapid human metapneumovirus microneutralization assay based on green fluorescent protein expression. J Virol Methods. 2005;128(1–2):192–197
  8. Anderson LJ, Hierholzer JC, Bingham PG, Stone YO. Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay. J Clin Microbiol. 1985;22(6):1050–1052
  9. Falsey AR, Walsh EE. Safety and immunogenicity of a respiratory syncytial virus subunit vaccine (PFP-2) in ambulatory adults over age 60. Vaccine. 1996;14(13):1214–1218
  10. Siber GR, Leszcynski J, Pena-Cruz V, et al. Protective activity of a human respiratory syncytial virus immune globulin prepared from donors screened by microneutralization assay. J Infect Dis. 1992;165(3):456–463
  11. van den Hoogen BG, Herfst S, Sprong L, et al. Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis. 2004;10(4):658–666
  12. Bastien N, Normand S, Taylor T, et al. Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains. Virus Res. 2003;93:51–62
  13. Biacchesi S, Skiadopoulos MH, Boivin G, et al. Genetic diversity between human metapneumovirus subgroups. Virology. 2003;315:1–9
  14. Skiadopoulos MH, Biacchesi S, Buchholz UJ, et al. The two major human metapneumovirus genetic lineages are highly related antigenically, and the fusion (F) protein is a major contributor to this antigenic relatedness. J Virol. 2004;78(13):6927–6937
  15. Skiadopoulos MH, Biacchesi S, Buchholz UJ, et al. Individual contributions of the human metapneumovirus F, G, and SH surface glycoproteins to the induction of neutralizing antibodies and protective immunity. Virology. 2006;345(2):492–501
  16. Walsh EE, Falsey AR. Age related differences in humoral immune response to respiratory syncytial virus infection in adults. J Med Virol. 2004;73(2):295–299

PII: S1386-6532(09)00436-3

doi: 10.1016/j.jcv.2009.09.020

Journal of Clinical Virology
Volume 46, Issue 4 , Pages 314-317 , December 2009

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