Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay
Received 3 June 2009; received in revised form 1 October 2009; accepted 7 October 2009. published online 09 November 2009.
Abstract
Background
Novel serological methods provide alternative options for sero-diagnosis, sero-epidemiology and for determining evidence of naturally acquired or vaccine induced immunity. Micro-neutralization tests are currently the gold standard for serological studies of highly pathogenic avian influenza in mammalian species but require handling live virus in a biosafety level (BSL) 3 environment. We previously reported the use of H5 pseudotyped lentiviral particles (H5pp) as an alternative to micro-neutralization tests in a BSL-2 setting (Nefkens et al., 2007).
Objective
To optimize and evaluate this newly developed H5pp assay on relevant clinical specimens.
Study design
We optimise and evaluate the performance of the H5pp assay using well-characterized sera from humans with confirmed H5N1 disease or controls.
Results
The H5pp assay is a reliable serological method for the detection and quantification of neutralizing antibody to H5-viruses.
Conclusion
H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting.
ABSTRACT