Journal of Clinical Virology
Volume 47, Issue 1 , Pages 49-53 , January 2010

Early diagnosis of dengue in travelers: Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology

  • Eili Huhtamo

      Affiliations

    • Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Finland
    • Corresponding Author InformationCorresponding author. Tel.: +358 9 19126706; fax: +358 9 19126491.
    • ,
  • Essi Hasu

      Affiliations

    • Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Finland
    • ,
  • Nathalie Y. Uzcátegui

      Affiliations

    • Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Finland
    • ,
  • Elina Erra

      Affiliations

    • Helsinki University Central Hospital, Department of Medicine, Division of Infectious Diseases, Helsinki, Finland
    • ,
  • Simo Nikkari

      Affiliations

    • Centres for Military Medicine and for Biological Threat Preparedness, Tukholmankatu 8A, 00290 Helsinki, Finland
    • ,
  • Anu Kantele

      Affiliations

    • Helsinki University Central Hospital, Department of Medicine, Division of Infectious Diseases, Helsinki, Finland
    • ,
  • Olli Vapalahti

      Affiliations

    • Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Finland
    • Department of Virology, HUSLAB, Helsinki University Central Hospital Laboratory, P.O. Box 400 (Haartmaninkatu 3), FI-00029 HUS, Finland
    • Division of Microbiology and Epidemiology, Department of Basic Veterinary Sciences, P.O. Box 66 (Agnes Sjöbergin katu 2), FI-00014 University of Helsinki, Finland
    • ,
  • Heli Piiparinen

      Affiliations

    • Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, P.O. Box 21 (Haartmaninkatu 3), FI-00014 University of Helsinki, Finland

Received 18 August 2009 ,Accepted 4 November 2009.

References 

  1. World Health Organization. Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd ed.; 1997.
  2. Rigau-Perez JG, Clark GG, Gubler DJ, Reiter P, Sanders EJ, Vorndam AV. Dengue and dengue haemorrhagic fever. Lancet. 1998;352(September (9132)):971–977
  3. Jelinek T, Muhlberger N, Harms G, Corachan M, Grobusch MP, Knobloch J, et al. Epidemiology and clinical features of imported dengue fever in Europe: sentinel surveillance data from TropNetEurop. Clin Infect Dis. 2002;35(November (9)):1047–1052
  4. Jelinek T. Trends in the epidemiology of dengue fever and their relevance for importation to Europe. Euro Surveill. 2009;14(June (25)):19250
  5. Shu PY, Huang JH. Current advances in dengue diagnosis. Clin Diagn Lab Immunol. 2004;11(July (4)):642–650
  6. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30(March (3)):545–551
  7. Johnson BW, Russell BJ, Lanciotti RS. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol. 2005;43(October (10)):4977–4983
  8. Dumoulin A, Marti H, Panning M, Hatz C, Hirsch HH. Pan-dengue virus detection by PCR for travelers returning from the tropics. J Clin Microbiol. 2008;46(September (9)):3104–3106
  9. Callahan JD, Wu SJ, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, et al. Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol. 2001;39(November (11)):4119–4124
  10. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002;40(February (2)):376–381
  11. Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, et al. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vac Immunol. 2006;13(November (11)):1185–1189
  12. Young PR, Hilditch PA, Bletchly C, Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol. 2000;38(March (3)):1053–1057
  13. Lemmer K, Donoso Mantke O, Bae HG, Groen J, Drosten C, Niedrig M. External quality control assessment in PCR diagnostics of dengue virus infections. J Clin Virol. 2004;30(August (4)):291–296
  14. Vene S, Mangiafico J, Niklasson B. Indirect immunofluorescence for serological diagnosis of dengue virus infections in Swedish patients. Clin Diagn Virol. 1995;4(July (1)):43–50
  15. Huhtamo E, Uzcategui NY, Siikamaki H, Saarinen A, Piiparinen H, Vaheri A, et al. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg Infect Dis. 2008;14(January (1)):80–83
  16. Scaramozzino N, Crance JM, Jouan A, DeBriel DA, Stoll F, Garin D. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol. 2001;39(May (5)):1922–1927
  17. Halstead SB, Dengue . Lancet. 2007;370(November (9599)):1644–1652
  18. Kumarasamy V, Chua SK, Hassan Z, Wahab AH, Chem YK, Mohamad M, et al. Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. Singapore Med J. 2007;48(July (7)):669–673

PII: S1386-6532(09)00542-3

doi: 10.1016/j.jcv.2009.11.001

Journal of Clinical Virology
Volume 47, Issue 1 , Pages 49-53 , January 2010

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