Journal of Clinical Virology
Volume 47, Issue 1 , Pages 4-7 , January 2010

Correlation between HIV-1 viral load quantification in plasma, dried blood spots, and dried plasma spots using the Roche COBAS Taqman assay

  • Mauro Andreotti

      Affiliations

    • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • Equally contributing to the work.
    • ,
  • Maria Pirillo

      Affiliations

    • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • Equally contributing to the work.
    • ,
  • Giovanni Guidotti

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • ,
  • Susanna Ceffa

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • ,
  • Giovanna Paturzo

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • ,
  • Paola Germano

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • ,
  • Richard Luhanga

      Affiliations

    • DREAM Program, Community of S. Egidio, Blantyre, Malawi
    • ,
  • David Chimwaza

      Affiliations

    • DREAM Program, Community of S. Egidio, Lilongwe, Malawi
    • ,
  • Maria Grazia Mancini

      Affiliations

    • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • ,
  • Maria Cristina Marazzi

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • Department of Public Health, LUMSA University, Rome, Italy
    • ,
  • Stefano Vella

      Affiliations

    • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • ,
  • Leonardo Palombi

      Affiliations

    • DREAM Program, Community of S. Egidio, Rome, Italy
    • Department of Public Health, University of Tor Vergata, Rome, Italy
    • ,
  • Marina Giuliano

      Affiliations

    • Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 06 49903303; fax: +39 06 49387199.

Received 18 August 2009 ,Revised 4 November 2009 ,Accepted 5 November 2009.

References 

  1. World Health Organization – UNAIDS – UNICEF. Towards universal access. Scaling up priority HIV/AIDS interventions in the health sector. World Health Organization 2008. Accessed July 14, 2009.
  2. Ferradini L, Jeannin A, Pinoges L, Odhiambo D, Mankhambo L, Karungi G, et al. Scaling up of highly active antiretroviral therapy in a rural district of Malawi: an effectiveness assessment. Lancet. 2006;367:1335–1342
  3. Chaiwarith R, Wachirakaphan C, Kotarathititum W, Prapartanaphan J, Sirisanthana T, Supparatpinyo K. Sensitivity and specificity of using CD4+ measurement and clinical evaluation to determine antiretroviral treatment failure in Thailand. Int Infect Dis. 2007;11:413–416
  4. Cassol S, Gill MJ, Pilon R, Cormier M, Voigt RF, Willoughby , et al. Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper. J Clin Microbiol. 1997;35:2795–2801
  5. Fiscus SA, Brambilla D, Grosso L, Schock J, Cronin M. Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper. J Clin Microbiol. 1998;36:258–260
  6. Mwaba P, Cassol S, Nunn A, Pilon R, Chintu C, Janes M, et al. Whole blood versus plasma spots for measurement of HIV-1 viral load in HIV-infected African patients. Lancet. 2003;36:2067–2068
  7. Brambilla D, Jenning C, Aldrovrandi G, Bremer J, Comeau AM, Cassol SA, et al. Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision, and RNA stability. J Clin Microbiol. 2003;41:1888–1893
  8. Uttayamakul S, Likanonsakul S, Sunthornkachit R, Kuntiranont K, Louisirirotchanakul S, Chaovavanich A, et al. Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection. J Virol Meth. 2005;128:128.134
  9. Garrido C, Zahonero N, Corral A, Arredondo M, Soriano V, de Mendoza C. Correlation between human immunodeficiency virus type 1 (HIV-1) RNA measurements obtained with dried blood spots and those obtained with plasma by use of Nuclisens EasyQ HIV-1 and Abbot RealTime HIV load tests. J Clin Microbiol. 2009;47:1031–1036
  10. Waters L, Kambugu A, Tibenderana H, Meya D, John L, Mandalia S, et al. Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda. J Acq Immune Def Synd. 2007;46:590–593
  11. Monleau M, Montavon C, Laurent C, Segondy M, Montes B, Delaporte E, et al. Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing. J Clin Microbiol. 2009;47:1107–1118
  12. Marconi A, Balestrieri M, Comastri G, Pulvirenti FR, Gennari W, Tagliazucchi S, et al. Evaluation of the Abbott real-time HIV-1 quantitative assay with dried blood spot specimens. Clin Microbiol Infect. 2009;15:93–97
  13. Travers SA, Clewley JP, Glynn JR, Fine PE, Crampin AC, Sibande F, et al. Timing and reconstruction of the most recent ancestor of the subtype C clade of human immunodeficiency virus type 1. J Virol. 2004;78:10501–10506
  14. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;i:307–310
  15. Kane CT, Ndiaye HD, Diallo S, Ndiaye I, Wade AS, Diaw PA, et al. Quantitation of HIV-1-RNA in dried blood spots by the real-time NucliSENS EasyQ HIV-1 assay in Senegal. J Virol Meth. 2008;148:291–295
  16. Alvarez-Munoz MT, Zaragoza-Rodriguez 1 S, Rojas-Montes O, Palacios-Saucedo G, Vazquez-Rosales G, Gomez-Delgado , et al. High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions. Arch Med Res. 2005;36:382–386

PII: S1386-6532(09)00547-2

doi: 10.1016/j.jcv.2009.11.006

Journal of Clinical Virology
Volume 47, Issue 1 , Pages 4-7 , January 2010

Article Tools

* Image Usage & Resolution