Journal of Clinical Virology
Volume 53, Issue 2 , Pages 106-109 , February 2012

Establishing diagnostic cut-off criteria for the COBAS AmpliPrep/COBAS TaqMan HIV-1 Qualitative test through validation against the Amplicor DNA test v1.5 for infant diagnosis using dried blood spots

Received 14 September 2011 ,Revised 28 November 2011 ,Accepted 1 December 2011.

References 

  1. Roche® COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Qual Test [package insert]. Branchburg, NJ: Roche; 2007;
  2. Stevens W, Erasmus L, Moloi M, Taleng T, Sarang S. Performance of a novel human immunodeficiency virus (HIV) type 1 total nucleic acid-based real-time PCR assay using whole blood and dried blood spots for diagnosis of HIV in infants. J Clin Microbiol. 2008;46(December (12)):3941–3945
  3. Uttayamakul S, Likanonsakul S, Sunthornkachit R, Kuntiranont K, Louisirirotchanakul S, Chaovavanich A, et al. Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection. J Virol Methods. 2005;128(September (1–2)):128–134
  4. Leelawiwat W, Young NL, Chaowanachan T, Ou CY, Culnane M, Vanprapa N, et al. Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand. J Virol Methods. 2009;155(February (2)):109–117
  5. Jacob SM, Anitha D, Vishwanath R, Parameshwari S, Samuel NM. The use of dried blood spots on filter paper for the diagnosis of HIV-1 in infants born to HIV seropositive women. Indian J Med Microbiol. 2008;26(January–March (1)):71–74
  6. Driver GA, Patton JC, Moloi J, Stevens WS, Sherman GG. Low risk of contamination with automated and manual excision of dried blood spots for HIV DNA PCR testing in the routine laboratory. J Virol Methods. 2007;146(December (1–2)):397–400
  7. Roche® product bulletin No. 2007/281. Branchburg, NJ: Roche; 2007;
  8. Violari A, Cotton MF, Gibb DM, Babiker AG, Steyn J, Madhi SA, et al. Early antiretroviral therapy and mortality among HIV-infected infants. N Engl J Med. 2008;359(November (21)):2233–2244
  9. Zanchetta M, Anselmi A, Vendrame D, Rampon O, Giaquinto C, Mazza A, et al. Early therapy in HIV-1-infected children: effect on HIV-1 dynamics and HIV-1-specific immune response. Antivir Ther. 2008;13(1):47–55
  10. Lima V, Harrigan R, Montaner JS. Increased reporting of detectable plasma HIV-1 RNA levels at the critical threshold of 50 copies per milliliter with the Taqman assay in comparison to the Amplicor assay. J Acquir Immune Defic Syndr. 2009;51(May (1)):3–6
  11. Mercier B, Burlot L, Férec C. Simultaneous screening for HBV DNA and HCV RNA genomes in blood donations using a novel TaqMan PCR assay. J Virol Methods. 1999;77(January (1)):1–9
  12. Shearer WT, Quinn TC, LaRussa P, Lew JF, Mofenson L, Almy S, et al. Viral load and disease progression in infants infected with human immunodeficiency virus type 1. Women and Infants Transmission Study Group. N Engl J Med. 1997;336(May (19)):1337–1342
  13. Dickover RE, Dillon M, Leung KM, Krogstad P, Plaeger S, Kwok S, et al. Early prognostic indicators in primary perinatal human immunodeficiency virus type 1 infection: importance of viral RNA and the timing of transmission on long-term outcome. J Infect Dis. 1998;178(August (2)):375–387

PII: S1386-6532(11)00506-3

doi: 10.1016/j.jcv.2011.12.002

Journal of Clinical Virology
Volume 53, Issue 2 , Pages 106-109 , February 2012

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