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Editors’ Introduction
17 October 2011
Currently in the United States the diagnosis of HIV-1 or HIV-2 infection is based on a testing algorithm that includes an initial screening with an enzyme immunoassay (EIA) or a rapid HIV test that de...
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Christine C. Ginocchio,
William F. Carman,
Max Q. Arens
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S1
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Establishing the diagnosis of HIV infection: New tests and a new algorithm for the United States
12 October 2011
With the introduction of third generation antibody assays that detect both immunoglobulin (Ig) G and IgM class antibodies, and fourth generation antigen/antibody (Ag/Ab) combination assays, the abilit...
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Bernard M. Branson,
Jonathan Mermin
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S3-S4
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Performance of an alternative laboratory-based algorithm for HIV diagnosis in a high-risk population
24 October 2011
Abstract: Background: An immunoassay (IA) followed by Western blot (WB) or immunofluorescence assay has been the primary algorithm used to provide laboratory confirmation of the diagnosis of HIV infec...
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Kevin P. Delaney,
James D. Heffelfinger,
Laura G. Wesolowski,
S. Michele Owen,
William A. Meyer,
Susan Kennedy,
Apurva Uniyal,
Peter R. Kerndt,
Bernard M. Branson
et al.
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S5-S10
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Use of a rapid HIV testing algorithm to improve linkage to care
10 October 2011
Abstract: Background: Awaiting definitive diagnosis before scheduling healthcare visits complicates HIV screening and referral. Clients screened by rapid tests as initially reactive often fail to retu...
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Eugene G. Martin,
Gratian Salaru,
Sindy M. Paul,
Evan M. Cadoff
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S11-S15
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Evaluation of an alternative HIV diagnostic algorithm using specimens from seroconversion panels and persons with established HIV infections
07 October 2011
Abstract: Background: The current algorithm for HIV diagnosis in the US involves screening with an immunoassay (IA) and supplemental testing with Western blot (WB) or immunofluorescence assay. Because...
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Silvina Masciotra,
J. Steven McDougal,
Jane Feldman,
Patrick Sprinkle,
Laura Wesolowski,
S. Michele Owen
et al.
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S17-S22
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Comparison of alternative interpretive criteria for the HIV-1 Western blot and results of the Multispot HIV-1/HIV-2 Rapid Test for classifying HIV-1 and HIV-2 infections
13 October 2011
Abstract: Background: HIV-1 Western blot (WB) may be positive in specimens from persons with HIV-2 infection due to cross-reactive antibodies. HIV-1 and HIV-2 infections may be identified using assays...
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Muazzam Nasrullah,
Steven F. Ethridge,
Kevin P. Delaney,
Laura G. Wesolowski,
Timothy C. Granade,
Joe Schwendemann,
Robert D. Boromisa,
James D. Heffelfinger,
S. Michele Owen,
Bernard M. Branson
et al.
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S23-S27
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Cost effectiveness analysis of the New Jersey rapid testing algorithm for HIV testing in publicly funded testing sites
14 November 2011
Abstract: Background: Before 2009, New Jersey (NJ) publicly funded counseling and testing sites (CTS) tested for HIV using a single rapid test followed, when positive, by a Western Blot (WB) for confi...
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Kendall Stevinson,
Eugene G. Martin,
Stephen Marcella,
Sindy M. Paul
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S29-S33
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Evaluation of an alternative supplemental testing strategy for HIV diagnosis by retrospective analysis of clinical HIV testing data
24 October 2011
Abstract: Background: Because newer screening assays are more sensitive than traditional confirmatory assays, a new HIV testing algorithm was proposed by the Centers for Disease Control and Prevention...
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Linda M. Styer,
Timothy J. Sullivan,
Monica M. Parker
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S35-S40
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Comparison of Multispot EIA with Western blot for confirmatory serodiagnosis of HIV
14 October 2011
Abstract: Background: Recent improvements in the sensitivity of immunoassays (IA) used for HIV screening, coupled with increasing recognition of the importance of rapid point-of-care testing, have led...
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Lucia V. Torian,
Lisa A. Forgione,
Amado E. Punsalang,
Robert E. Pirillo,
William R. Oleszko
et al.
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S41-S44
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Performance of an alternative laboratory-based algorithm for diagnosis of HIV infection utilizing a third generation immunoassay, a rapid HIV-1/HIV-2 differentiation test and a DNA or RNA-based nucleic acid amplification test in persons with established HIV-1 infection and blood donors
13 October 2011
Abstract: Background: The HIV-1 Western blot (WB) and immunofluorescence assay used to confirm HIV infections are less sensitive during seroconversion than immunoassays (IAs) used for screening. An al...
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Laura G. Wesolowski,
Kevin P. Delaney,
Clyde Hart,
Carolyn Dawson,
S. Michele Owen,
Debra Candal,
William A. Meyer,
Steven F. Ethridge,
Bernard M. Branson
et al.
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S45-S49
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Evaluation of the performance of the Abbott ARCHITECT HIV Ag/Ab Combo Assay
10 October 2011
Abstract: Background: Worldwide, many countries test for HIV infection using combination assays that simultaneously detect p24 antigen and HIV antibodies. One such assay, the ARCHITECT® HIV Ag/Ab Comb...
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Pollyanna Chavez,
Laura Wesolowski,
Pragna Patel,
Kevin Delaney,
S. Michele Owen
et al.
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S51-S55
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Performance evaluation of the Bio-Rad Laboratories GS HIV Combo Ag/Ab EIA, a 4th generation HIV assay for the simultaneous detection of HIV p24 antigen and antibodies to HIV-1 (groups M and O) and HIV-2 in human serum or plasma
13 October 2011
Abstract: Background: A multi-center study was conducted to evaluate the Bio-Rad GS HIV Combo Ag/Ab EIA, a 4th generation HIV-1/HIV-2 assay for the simultaneous detection of HIV p24 antigen and antibo...
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Christopher Bentsen,
Lisa McLaughlin,
Elizabeth Mitchell,
Carol Ferrera,
Sally Liska,
Robert Myers,
Sheila Peel,
Paul Swenson,
Stephane Gadelle,
M. Kathleen Shriver
et al.
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S57-S61
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Comparative evaluation of Aptima HIV-1 Qualitative RNA assay performance using plasma and serum specimens from persons with established HIV-1 infection
14 October 2011
Abstract: Background: Blood specimens for HIV testing are frequently collected using serum collection tubes. The Aptima HIV-1 RNA Qualitative test, originally approved for diagnostic use as a suppleme...
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Steven F. Ethridge,
Laura G. Wesolowski,
Muazzam Nasrullah,
M. Susan Kennedy,
Kevin P. Delaney,
Debra Candal,
S. Michele Owen
et al.
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S63-S66
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Ten years of HIV testing with fourth generation assays: The Amsterdam experience
13 October 2011
Abstract: Serological HIV assays combining detection of HIV antigen and antibodies are referred to as fourth generation assays. Fourth generation assays were implemented in Europe for routine patient ...
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Suzanne Jurriaans,
Nicole K.T. Back,
Katja C. Wolthers
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S67-S69
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HIV testing—The perspective from across the pond
17 October 2011
Abstract: The first assay to screen blood donations for HIV was licensed in the USA in 1985. Since then there has been a dramatic increase in the types and numbers of assays available for HIV testing ...
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Gary Murphy,
Celia Aitken
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S71-S76
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Evolution in the sensitivity of quantitative HIV-1 viral load tests
31 October 2011
Abstract: Significant advancements in molecular diagnostics have been made since the inception and application of PCR-based technologies in clinical diagnostic laboratories and the management of HIV-1...
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Bryan R. Cobb,
Jeffrey E. Vaks,
Tri Do,
Regis A. Vilchez
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S77-S82
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Comparative frequencies of HIV low-level viremia between real-time viral load assays at clinically relevant thresholds
13 October 2011
Abstract: Background: The introduction of new real-time PCR HIV-1 assays with higher sensitivity and broader dynamic range has resulted in detection of low-level viremia (LLV) (≥50copies/mL) in some p...
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Tri Do,
John Duncan,
Ann Butcher,
Teri Liegler
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S83-S89
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